Testing for color changes when iron(II) sulfate solution is dripped onto the specimen
Observation | FeSO4 (aq) Test |
---|---|
Photos / SoundsWhatOnion-stalk Parasol (Leucocoprinus cepistipes)ObservermycomutantDescriptionSpores: (6) 7-14 (15) x (5) 6-8 (9) µm. Ellipsoid, amygaldiform or subglobose. Hyaline, smooth and with a large greenish oil droplet/guttule inside and a capped germ pore. Dextrinoid, congophilous, cyanophilous, metachromatic in Cresyl blue with pinkish purple inner wall. Very slight pinkish colour in 1% Aniline blue in lactic acid. Pinkish colour to inner spore and yellowish tone to wall observed in 5% KOH mount becoming more noticeable in 10%. Guttules were mostly single, some double or triple. The largest single ones observed measured: 3-4 x 3-4.5 µm. Dried mushroom cap showed a very slight pinkish tone when 5% KOH was applied, though mostly just bleached. 10% FeSO4 resulted in similar bleaching but no colouration was noted. Leucocoprinus species sent to me from a Reddit user in the UK. Growing in a potted Aspidistra plant inside. Presumably Aspidistra elatior. https://www.reddit.com/r/mycology/comments/151e3sn/id_for_these_fellas_uk/ Spore prints and a number of dried specimens were received - the gills of which had discoloured to such a greenish grey colour that they initially looked like they could be moldy, since they had rehydrated slightly in the mail. Upon closer inspection however this was just discolouration, or possibly was the result of the greenish guttules/oil droplets inside the spores. The spore print appeared whitish on the black paper but the slides with gill sections mounted showed a slightly greenish grey colour where spores were spilling out, visible with the naked eye. So perhaps enough spore mass in a print would have the same tone. Macroscopically the mushrooms appear similar to Leucocoprinus cepistipes with the distinctive looking shape of the immature mushrooms complete with the clear exudation. I have seen a similar greenish grey gill discolouration in observations before, see here: https://www.inaturalist.org/observations/20082876 https://www.inaturalist.org/observations/32393475 Whereas other times a more reddish pink, brownish or yellowish tone is present. I'm not clear if there is any distinction between these or if it just represents different stages of discolouration. Microscopically however the spores were far larger than those noted in descriptions of L. cepistipes. A size of (6) 7-14 (15) x (5) 6-8 (9) µm was observed from spores spilling out of a gill section crushed under the cover glass. From the spore print however a smaller size was observed of 7-11 x 6-7 µm. The larger spores were immediately obvious upon looking at a gill section and were not just outliers. Flora Agaricina Neerlandica vol. 5 gives a spore size for L. cepistipes of 7.5 - 13 x 6-8 µm so this would seem out of that range. I have also observed this with L. ianthinus where a gill section results in a larger spore size reading than from the print. So it appears that measuring the spore size of Leucocoprinus species from prints may not be reliable. I have not read anything to the effect of spore prints being less reliable than gill section mounts for measuring spore size. Mushroom Expert seems to note the opposite, mentioning that immature spores may be seen with a gill mount. Spores that appeared obviously immature and undeveloped weren't included in my measurements, but seemed fewer than the large spores anyway. Most of the spores with a measurment of 5µm were closer to spherical and either 5x5 or 6x5. They appeared fully formed though rather than immature. I am speculating that perhaps Leucocoprinus species retain more of the large spores when they are found outside of high humidity, tropical environments as a larger buller's drop would need to form in order to release larger spores. However I've never looked for this phenomenon in common, native garden mushrooms so will have to see if I record the same thing there. Comparing the photos of the mushrooms and the size of the spores to the 80 odd species of Leucocoprinus for which I have adequate information I cannot find a direct match. My best guess currently is that this may be something that has been described as a variant or form of L. cepistipes. So I'm going to try and get the description for Leucocoprinus cepistipes f. macrosporus Migl. to see if this may match and explain the larger spores. They appear similar to this observation from New Zealand, also found in an Aspidistra plant so perhaps the plant may point to an origin for this species/variant. Will need to look for more observations with these plants. |
bleaching |
Photos / SoundsWhatPale Brittlestem (Candolleomyces candolleanus)ObservermycomutantDescriptionA cluster of mushrooms on a log bench in the woods. Some years ago I found Rhodotus palmatus growing on the bench so went to look for it but found these instead, so I presume the logs to be elm. Most of the mushrooms had been eaten but one relatively intact one was possible to try and identify and appears to be Candolleomyces. Spore size seems to match Candolleomyces candolleanus at 6.5-8 x 4 - 5μm. KOH and FeSO4 produced no noticeable change or a bleaching reaction. |
bleaching |
Indistinct | |
Photos / SoundsWhatSticky Scalycap (Pholiota gummosa)ObservermycomutantDescriptionGrowing near trees. There was a major fire in the area during the summer heatwave so there is much burnt wood and blackened soil around. However these didn't specifically seem to be growing from the burnt areas. Reddish yellow reaction to KOH, redder on the scales and exposed flesh. Yellowish reaction to FeSO4. Spore print not tested for these as there were only two growing so I left them alone. |
Yellowish |
Photos / SoundsWhatUpright Coral Fungus (Ramaria stricta)ObservermycomutantDescriptionSmall patch of Ramaria growing beside footpath in deciduous woodland. They've been there for some months now. Wasn't intending to collect this one but it broke when I touched it so I thought I would make the most of it. Pleasant smell but can't really describe it. Reddish brown reaction on flesh to KOH and greenish blue with FeSO4. Spores are yellow in Melzers, colourless without. Spores: 6.5 - 8.5 x 3 - 4.5μm. Basidia 4 spored. Note: in situ photos are from early October whilst microscopy is from early November (I forgot to take more photos of them recently but they still look the same). |
Greenish blue |
Photos / SoundsWhatSticky Scalycap (Pholiota gummosa)ObservermycomutantDescriptionOn mossy ground in a dry pond bed from the old water treatment works. The area is in a drought at the moment so this is usually underwater and just full of bullrushes and reeds. There is a grey willow growing nearby though that is usually partially submerged. Reddish yellow reaction to KOH, redder on the scales and exposed flesh. Yellowish reaction to FeSO4. Brownish spore print (photo from two days later). In dry pond bed near Gutteridge Wood. Edit: 11/10/23 Added microscopy (forgot I had these spore prints lying around). Spores: (5.5) 6-7 (8) x (3) 3.5-4 (5) µm. Ellipsoid and smooth with a germ pore. Light pinkish in colour with yellow guttules. In Mezler's the entire spore becomes brighter yellow. |
Yellow |
Photos / SoundsWhatBeefsteak Polypore (Fistulina hepatica)ObservermycomutantDescriptionBeef steak fungus fallen/removed from tree. It was not entirely clear what this was at first and so I didn't simply turn it over but rather proceeded to examine it as if it was attached to the ground and test as if it were a Bolete... Dark reddish reaction to KOH on cap and pores. Greenish with FeSO4. However the specimen a few feet away made it obvious that this was also just a beef steak. |
Green |
Photos / SoundsWhatSulphur Tuft (Hypholoma fasciculare)ObservermycomutantDescriptionGrowing in a large cluster on a rotting tree stump (likely oak) and fallen wood beneath a yew tree. Reddish reaction to KOH. Greenish to FeSO4. Ten Acre Wood. |
Green |
Photos / SoundsWhatSpectacular Rustgill (Gymnopilus junonius)ObservermycomutantDescriptionGrowing in a dry stream/drainage ditch beside flood barriers. The area is technically still in a drought and the nearby river is low. Not as many mushrooms in these woods as there usually are this time of year. This ditch is usually full of water and this is the first time I've seen it dry enough to walk around. For the last few years I've found Gymnopilus junonius growing from a tree stump in the woods a couple hundred meters away but it is currently devoid of any and only hosts some brackets and Hypholoma. Strong, quick red reaction to KOH on cap surface and exposed flesh. Slight discoloration with FeSO4. Ten Acre Wood. Same specimens three days later: |
Darkish, indistinct |
Photos / SoundsWhatRollrims (Genus Paxillus)ObservermycomutantDescriptionSmall trooping group growing beneath a grey willow on mossy ground. The area is usually underwater as it is a pond from the old water treatment works. The willow has exposed roots on the surface from being partially submerged normally. However there is a drought at the moment and the pond is dry so it was accessible to walk across and explore. Strong red reaction to KOH and greenish reaction to FeSO4. In dry pond bed near Gutteridge Wood. |
Green |
Cap Surface: Blue/Green ... Cap Flesh: Blue/Green ... Stem Surface: Blue/Green ... Stem Flesh: Blue/Green | |
Cap Surface: - ... Cap Flesh: - ... Stem Surface: - ... Stem Flesh: - | |
Photos / SoundsWhatMushrooms, Bracket Fungi, Puffballs, and Allies (Class Agaricomycetes)Observerfourkilorouenne |
Negative |
Photos / SoundsWhatMilkcaps, Brittlegills and Allies (Family Russulaceae)Observerfourkilorouenne |
Cap Surface: - ... Cap Flesh: - ... Stem Surface: - ... Stem Flesh: - |
Cap Surface: - ... Cap Flesh: Blue/Black ... Stem Surface: - ... Stem Flesh: - | |
Cap Surface: - ... Cap Flesh: Blue ... Stem Surface: - ... Stem Flesh: - | |
Cap Surface: - ... Cap Flesh: Green/Gray ... Stem Surface: - ... Stem Flesh: - | |
Photos / SoundsWhatMilkcaps, Brittlegills and Allies (Family Russulaceae)Observerfourkilorouenne |
Cap Surface: - ... Cap Flesh: Green/Gray ... Stem Surface: - ... Stem Flesh: Green/Gray |
Cap Surface: - ... Cap Flesh: - ... Stem Surface: - ... Stem Flesh: - | |
Cap Surface: - ... Cap Flesh: - ... Stem Surface: - ... Stem Flesh: - | |
Cap Surface: - ... Cap Flesh: - ... Stem Surface: - ... Stem Flesh: - | |
Cap Surface: - ... Cap Flesh: - ... Stem Surface: - ... Stem Flesh: - | |
Cap Surface: - ... Cap Flesh: - ... Stem Surface: - ... Stem Flesh: - | |
Cap Surface: - ... Cap Flesh: Green ... Stem Surface: - ... Stem Flesh: Green | |
Cap Surface: Blue-Green ... Cap Flesh: Blue ... Stem Surface: Blue ... Stem Flesh: Blue | |
Cap Surface: - ... Cap Flesh: Yellow ... Stem Surface: - ... Stem Flesh: Yellow | |
Photos / SoundsWhatBoletes and Allies (Order Boletales)ObserverfourkilorouenneDescriptionShape looks like Boletinellus merulioides but the trees here were mostly oak and hemlock |
Cap Surface: - ... Cap Flesh: Brown ... Stem Surface: - ... Stem Flesh: Brown |