CR PE APOCYNACEAE Asclepias dissona
This species was last recorded in 1932. In spite of extensive survey efforts by the Mpumalanga Parks and Tourism Agency, this species has not been found. Its habitat is extensively degraded and it is possibly extinct.
Full Red List Account

EX MYRTACEAE Eugenia pusilla Amsterdam Myrtle
Last collected in 1920, and never seen again despite numerous searches. The area where it was last seen have been transformed to wattle plantations.
full red list account

CR PE ASPHODELACEAE Kniphofia crassifolia
Last seen in 1880. More than 80% of the grasslands in the Woodbush area, where this species presumably grew, has been converted to commercial pine plantations since the type collection. Remnant fragments are severely degraded either due to too frequent fire, or to thick infestations of invasive alien plants. If, like many congeners, this species grew in marshy areas, the possibility of its persistence is particularly poor as pine plantations lower the water table in open grassland fragments. Searches in the area have failed to relocated this species.
Full Red List Account

CR PE APOCYNACEAE Orbea elegans
Described in 2004 from a single plant at a site that has subsequently been destroyed by livestock overgrazing and erosion. Searches in the area have not yet relocated any other surviving individuals. The site of another unconfirmed record seen in the 1960s was destroyed by dam construction.
full red list account

EW ZAMIACEAE Encephalartos brevifoliolatus Escarpment Cycad
When this species was described in 1996, there were thought to be five mature plants in one site in the Limpopo Province. Collectors removed most of the remaining plants and left only a few damaged stems. These were removed by conservation officials for ex situ conservation.
full red list account

EW ZAMIACEAE Encephalartos nubimontanus Blue Cycad
Surveys conducted in Limpopo province show a decline from 66 plants in the 1990s, to eight plants in 2001 and no recorded plants in 2004. As with other cycad species in Limpopo province it was mostly impacted by illegal collecting.
full red list account

APOCYNACEAE Brachystelma canum
South African endemic, last collected in 1956. The locality is well-documented. Several attempts to relocate this species at the type locality and surrounding areas have been futile (Hahn 2013). It is possibly extinct due to habitat loss.

Reference: Hahn, N. 2013. Rare, endangered and endemic flora of the North West Province. Unpublished Report to the Department of Economic Development, Conservation and Tourism, North West Provincial Government.

APOCYNACEAE Miraglossum laeve
A very rare and poorly known species. It has been collected twice only: first in 1930, from hills south of Pretoria, and again in 1960, from the hills of the Vredefort Dome north-east of Parys, a disjunction of about 130 km. Despite dedicated searches, this species has not been found again (@sp_bester pers. comm.). The 1960 collection has a fairly precise locality description, which indicates that it occurs in Gold Reef Mountain Bushveld, a vegetation type with a limited distribution on the rocky ridges of Gauteng and adjacent areas in North West Province. The older collection's locality description is too imprecise to determine its habitat, but hills to the south of Pretoria also has another limited vegetation type on them, Gauteng Shale Mountain Bushveld, which is found on three ridges across Gauteng Province, and also extending somewhat into North West Province. It is therefore likely that this species is a rare, localized endemic of ridges in Gauteng Province.

Reference: http://redlist.sanbi.org/species.php?species=2707-3

ASTERACEAE Senecio holubii
only known from the type specimen, collected in 1876 in a remote area of South Africa near the border with Botswana. This area is botanically relatively poorly explored, and it may be overlooked. However, a recent field survey of rare, endangered, and endemic plants of North West province found the area to be severely degraded, and the species could not be relocated (Hahn 2013). The status and trend of the population is not known, and it is possibly extinct, but more field surveys are needed to confirm this.